Missouri S&T cDNA Resource Center

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Missouri S&T cDNA Resource Center

Is a non-profit service providing full-length cDNA clones encoding human signal transduction proteins to the research community. The distribution fee reflects our direct costs for producing, verifying, and distributing these clones.

The Center provides clones of human proteins that are:

Full-length
Sequence verified
Expression verified in most cases by coupled in vitro transcription/ translation assays
Propagated in versatile vectors (primarily the mammalian expression vector pcDNA3.1+, Invitrogen)
Free of extraneous 3' and 5' untranslated regions
Available in wild-type, epitope-tagged and useful mutant forms (e.g., constitutively-active, dominant negative, PTX-resistant)
Shipped by courier delivery (Federal Express), most within a day of placing your order
Guaranteed for 6 months from original date of purchase

Contact Information

Order/Payment Questions: cDNA Resource Center staff members are available at (573) 341-7610 on Monday through Friday from 9:00am-2:00pm CST, via email (cdna@mst.edu), or by FAX (573) 341-7609.

Technical Assistance: Email is the preferred method for technical issues, or you may also call our Lab Manager directly at (573) 341-6988. Requests will receive a response within one business day.

Announcements

NEW PRODUCTS AVAILABLE:

1/26/12: GPR97

1/4/12: New versions available: RGS20 v2 3xHA-tagged (N-terminus), RalA 3xHA-tagged (N-terminus), RalB 3xHA-tagged (N-terminus)

10/25/11: GPR18, GPR32,GPR82, NPBWR1, OXER1, and RGS20 v2

Nuclear Receptor Clones: The cDNA Resource Center is pleased to announce the availability of a large set of clones for human nuclear receptors, including many undocumented variants. Clones are available for immediate delivery, and can be ordered using the catalog tree on the left.

Addtional information regarding nuclear receptors may be found at www.nursa.org.

Stable Transfected Cell lines: The cDNA Resource Center now offers stable transfected CHO-K1 cells with M1, M2, M3, M4 and M5 (muscarinic receptors 1-5) in both wild-type and 3xHA tagged (N-terminus) versions. These cell lines are $250 USD each, plus shipping. To protect the viability of the cells, orders must be received in our office by the close of business on Friday, to be shipped on the following Monday. Shipping cost will vary, and may not be available to all countries. Please contact us at cdna@mst.edu for additional information.

Special Development Service: If you are interested in a clone for human G protein, G protein regulator, GPCR, GPCR regulator, or small G protein, that is not in our collection, we may be able to help! We will develop a cDNA construct to your specifications for a flat fee of $500 USD (plus standard shipping). The availablity of this service, and time required for completion may vary; please contact us at cdna@mst.edu for more information.

Online Catalog Available as PDF

The cDNA Resource Center catalog is available for download via the "Download Catalog" link in the toolbar at the top of the screen.

Adobe Acrobat Reader is required to view the PDF file and is available free of charge from the Adobe Homepage: http://www.adobe.com/products/acrobat/readstep2.html

Shipping

Orders for cDNA will be charged $40 USD for international shipping by FedEx international priority. Domestic orders for cDNA are charged $15.50 for shipping FedEx overnight. Shipping cost for stable transfected cell lines may vary, and may not be available to all countries. Please contact us at cdna@mst.edu for more information.

It is the customer's responsibility to notify us of any requirements for import of biological materials, and to provide any necessary license or permits. Please inform us of any special instructions which we should observe to assist with a smooth passage of your order through customs.

Packaging

All clones developed for distribution are blotted as dried DNA in a 0.5ml screw-cap micro-centrifuge tube. These blots will be the same relative amount (~ 5000ng) dried on the side of the tube and then individually labeled. Instructions for re-suspending the dried DNA is on the FAQ provided with each order, and is also available under the FAQS heading on this website.

Contact Information

If you have not received a confirmation within 3 days of placing your order, or if you have any questions or concerns, please contact us at cdna@mst.edu.

cDNA Resource Center Staff

Important Notice: In any area in which the Terms and Conditions of the purchaser are in conflict with the Missouri S&T cDNA Resource Center's Material Transfer Agreement (MTA), or with the laws of the state of Missouri, the Missouri S&T cDNA Resource Center MTA and the laws of the state of Missouri will take precedence. Placing an order with the Missouri S&T cDNA Resource Center constitutes your agreement to these terms. The full MTA is available by clicking on the tab at the top of the page or the link within the body of this disclaimer.

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